Re: [DIYbio] That Canadian company (or part of a uni) that was making a magnetic bead plasmid assembly kit

Couldn't you just buy some primers of the same overhang and blunt ligate?

On Wed, Feb 19, 2014 at 1:30 PM, Cory Tobin <cory.tobin@gmail.com> wrote:
>> Dan, what do you mean by 'stuck with what is in the kit'?
>
> The parts aren't easily manufactured. The overhangs are purified via
> HPLC, so unless you have access to that you won't be able to make your
> own parts. Doug (from Genomikon) is on this list I think, so maybe he
> can provide insight about making Genomikon parts.
>
> That being said, it's a great kit especially for educational purposes.
> I've taught a couple classes using the kit and I think we've had 100%
> success rate in terms of the students getting at least one colony
> containing the correct plasmid. It's really foolproof and can be done
> in a couple of hours.
>
> I doubt it will be used in many research settings. If you're making
> one-off DNA constructs you're better off using traditional cloning
> methods. But I could see it being used in a situation where you have
> a small number of parts that you recombine in lots of different
> combinations, in which case it would save you a boat load of time.
>
> -cory
>
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-Nathan

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