Re: [DIYbio] Ultra-Cheap DNA Printing/Sequencing

you don't need some new kind of laser to do that though, you just need
the right optical know-how and a decent detector setup. Read the
patents by Helicos and you'll find plenty of similar setups in
academic literature:
https://groups.google.com/forum/#!topic/diybio/Ke7BqPUD46A

You can label-free detection by moving to a Raman setup.

On Fri, Feb 21, 2014 at 4:37 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Well, Sung (@bookhling) can do nano-lasers. I recall he did bio-nanodots
> and got them to lase. Crazyballs.
>
> On 22/02/14 00:32, John Griessen wrote:
>> On 02/21/2014 01:40 PM, L wrote:
>>> The resulting oglios would then be preferentially selected by
>>> sequencing all and destroying the ones that don't match the desired
>>> sequence.
>>>
>>> So basically, yes.
>>
>> But, nanofabbing of nano-lasers, or even lenses that can aim at a nano
>> speck of anything?
>> Not in my bag of tricks...
>>
>> Cathal Garvey wrote:
>>> An alternative reaction without chain-branching, which can be recovered
>>> as pure DNA using PCR, would be great.
>>
>> But, following a published method like,
>>
>> "I've seen at least one paper on this but lost it, and forgot the
>> keywords! - It used a DNA analogue
>> for the synthesis step, which was compatible enough with DNA Polymerases
>> that it could be PCR'd into real DNA afterwards."
>>
>> seems less imaginary. Thermo-cyclers we can do. Nano-lasers NOT (yet...)
>>
>
> --
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--
-Nathan

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