Hi everyone!
-- I want to do this:
Promoter CMV ---- GFP ---- Promoter EF1A ---- Neomycin Resistance ---- Terminator SV40
Is that ok with eukaryotic cells?
The CMV transcript will be Kozak -- GFP-- junk promoter ef1a -- junk neoR -- (and properly terminated)
The EF1a transcript will be Kozak -- NeoResistance --(and properly terminated)
It was said here that weak transcription termination may lead to silencing or other unwanted effects....
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