Btw,
On Monday, April 28, 2014 12:03:52 AM UTC+2, Mega [Andreas Stuermer] wrote:
-- There is no cheap way around it. Would add like 2000$ to change the vector, as it already contains the GFP.
I want to see GFP and NEO highly expressed
On Monday, April 28, 2014 12:03:52 AM UTC+2, Mega [Andreas Stuermer] wrote:
Hi everyone!I want to do this:Promoter CMV ---- GFP ---- Promoter EF1A ---- Neomycin Resistance ---- Terminator SV40Is that ok with eukaryotic cells?The CMV transcript will be Kozak -- GFP-- junk promoter ef1a -- junk neoR -- (and properly terminated)The EF1a transcript will be Kozak -- NeoResistance --(and properly terminated)It was said here that weak transcription termination may lead to silencing or other unwanted effects....
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