Re: [DIYbio] Finished and tested lightbulb pcr from bosslab- detailed build info included.

Nice work!

It would be helpful if you showed the final image of the tube here
with a piece of tape installed, it took me a while of clicking back
and forth between the different pages looking for the tubes
installed/fixed in place until I read "The proper way to mount samples
is by taping them inside the taper of the 2-4 coupler, as seen here"
but there isn't actually any tape there! Did you use black electrical
tape (absorbs IR well) or clear Scotch/cello tape?

http://tequals0.wordpress.com/2014/04/07/lightbulb-pcr-usage/


On Sun, Apr 6, 2014 at 10:36 PM, Avery louie <inactive.e@gmail.com> wrote:
> Hey All,
>
> Remember lightbulb pcr? Its not the best way of doing pcr, but it is cheap
> and the parts are easy to get. One issue I had with referring people to the
> build or the idea of a lightbulb pcr was a lack of working builds.
>
> In an effort to finish the project started at BOSSLAB, I brought the
> hardware home with me. I changed the sensor to a nice (and cheap) i2c
> sensor, and wrote some code.
>
> Using the 2x PCR mix generously donated by syd labs, I amplified some test
> DNA on the machine and ran it on one of my homebrew gel boxes (dox coming
> soon). It worked! Photo here with annotations of what lane is what.
>
> Build details, including BOM, schematic, source code and comments
>
> Usage guidelines and suggestions
>
> Follow my blog if you want updates on upcoming DIYBIO equipment builds.
> Things that are floating around in my mind right now to build: DNA
> quantifier/separator, a nice cheap peltier PCR, digital microfluidics...who
> knows?
>
> --A
>
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--
-Nathan

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