Re: [DIYbio] low cost bioreactor

In industry/academia as much as possible is autoclaved, otherwise
alcohol or phenol can be used to sterilize chemically, ozone (HEPA
filter blowing past an ionizer), or gamma radiation (this is how
sterile plastic culture flasks are sterilized). UV is used in
biocontainment hoods but its efficacy is argued (or so I've heard here
on this list). UV is sold at camping stores and for home well-water
treatment too, so it might not be 100%, but it does seem to cut down
enough that our immune systems can handle the rest (you don't have
that liberty in a bioreactor with no immune system).

On Mon, Apr 7, 2014 at 12:15 PM, John Griessen <john@industromatic.com> wrote:
> On 04/07/2014 01:07 PM, Open BioLab Graz wrote:
>>
>> building a totally sterile system to grow less strong bacteria/funghi is a
>> little different and ways more expensive.
>
>
> What sterilization methods are practical for a reactor with ports for
> stirring, pH,
> conductivity, gas bubbler, and a big cleanout opening? Is autoclaving
> necessary,
> or is UV light workable?
>
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--
-Nathan

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