Re: [DIYbio] My DIY Gene Gun worked!!


Hey. Super-nice.

Just one thing. Do you have a control? Because it really looks like some fluorescent dust fibers.

On Sat, Apr 19, 2014 at 9:17 PM, Meredith L. Patterson <clonearmy@gmail.com> wrote:
Sweet! Can't wait to read the writeup! (And if it's just plumbing parts and a bike pump, shit, I probably have all that lying around...)

Cheers,
--mlp


On Sat, Apr 19, 2014 at 8:00 PM, Sebastian Cocioba <scocioba@gmail.com> wrote:
Here is a picture of a plant leaf expressing gfp shot using tungsten nanoparticles using my home made gene gun. Ill write a decent post on my blog about it but I was too excited to wait. This was 48hrs post shot. Tobacco leaf, 35s-gfp-Tnos cassette. Left leaf is experiment, right leaf is control. Not the glow in the midrib. Leaf illuminated with party light black light.

Cost of gene gun was $30-50 depending on shipping (plumbing parts and bike pump), tungsten is $38/5g from US Nano, not counting the calcium or spermidine on looking at $0.01/shot (0.5mg) and all done at room temp!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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