Re: [DIYbio] My DIY Gene Gun worked!!

Here is a picture of a plant leaf expressing gfp shot using tungsten nanoparticles using my home made gene gun. Ill write a decent post on my blog about it but I was too excited to wait. This was 48hrs post shot. Tobacco leaf, 35s-gfp-Tnos cassette. Left leaf is experiment, right leaf is control. Not the glow in the midrib. Leaf illuminated with party light black light.

Cost of gene gun was $30-50 depending on shipping (plumbing parts and bike pump), tungsten is $38/5g from US Nano, not counting the calcium or spermidine on looking at $0.01/shot (0.5mg) and all done at room temp!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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