Hydrolysis of phosphodiesters has a half life of > 100,000 years in DNA.
I can't believe dNTPs degrade in 15 minutes at RT unless of course you know of some data to back this up but this is also off topic and besides the point as I said. It has been tested and there is no issue with shipping the master mixes.
On Sun, Apr 27, 2014 at 12:45 AM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
Tat's why you freeze the ligase buffer, btw. ATP is needed by the ligase
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