Re: [DIYbio] The Open Discovery Institute(ODIN) is Opening a DIYBio Store Soon!

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I don't have data to point either way, but phosphodiester bonds in DNA
are a 1:1 sugar:phosphate ratio, whereas ATP is 1:3, with the phosphates
daisy-chained directly to one another. There's a strong electrostatic
force pushing ATP apart, that's where the energy comes from when it's split.

So, I'd expect ATP, a very high-energy molecule, to have a much shorter
half-life than DNA even ignoring the redox potential of ATP outside of a
reductive cell environment.

On 27/04/14 09:11, Josiah Zayner wrote:
> Hydrolysis of phosphodiesters has a half life of > 100,000 years in DNA.
>
> I can't believe dNTPs degrade in 15 minutes at RT unless of course you know
> of some data to back this up but this is also off topic and besides the
> point as I said. It has been tested and there is no issue with shipping the
> master mixes.
>
>
> On Sun, Apr 27, 2014 at 12:45 AM, Mega [Andreas Stuermer] <
> masterstorm123@gmail.com> wrote:
>
>> Tat's why you freeze the ligase buffer, btw. ATP is needed by the ligase
>>
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T: @onetruecathal, @IndieBBDNA
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