Re: [DIYbio] Re: Z-competent cells

This is my favorite technique:
http://openwetware.org/wiki/TOP10_chemically_competent_cells
I didn't document the part about glaring at them.

On Apr 4, 2014, at 9:46 AM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:

> I'm told it's Tom Knight's "Second Favourite" method. Nobody told me
> what his favourite method is. I assume it's just glaring at recalcitrant
> cells until they transform themselves.
>
> On 04/04/14 03:04, Dakota Hamill wrote:
>> So have many people
>> On Apr 3, 2014 2:01 PM, "Mega [Andreas Stuermer]" <masterstorm123@gmail.com>
>> wrote:
>>
>>> Proprietary?
>>>
>>> Cathal's been using that for years :D
>>>
>>>
>>> Am Mittwoch, 2. April 2014 01:23:50 UTC+2 schrieb Joseph Chemler:
>>>>
>>>> Does anyone have a guess at the technology behind Zymo Research's
>>>> Z-competent cells? (It's proprietary)
>>>> They do not require heat shock and are super easy to make. Probably the
>>>> easiest plasmid transformation procedure I have ever used.
>>>>
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>
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