Why not maintain them in active culture? Not sure how to handle contamination since I've never worked with anything that's not green but maybe you can keep the tooth in a nutrient batch with antibiotics and somehow oxygenate it? Just a wild guess. I really have no clue so its all sci-fi to me but would be interested in what others who work with mammalian lines have to say.
Wouldn't any kind of freezing, without proper cryoprotectants, just end up killing the cells within the tooth?
How would one culture the cells effectively without an ECM scaffold to bind them? Can they grow as a suspension without much effort?
What kind of media would be required to maintain a tissue like that?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Wouldn't any kind of freezing, without proper cryoprotectants, just end up killing the cells within the tooth?
How would one culture the cells effectively without an ECM scaffold to bind them? Can they grow as a suspension without much effort?
What kind of media would be required to maintain a tissue like that?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Mega [Andreas Stuermer]
Sent: 6/5/2014 8:59 AM
To: diybio@googlegroups.com
Subject: [DIYbio] Cheaply freezing Wisdom tooth
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