Re: [DIYbio] Cheaply freezing Wisdom tooth

If you can't extract cells for proper preservation in FBS/DMSO, snap-freeze in nitrogen and store at -80, I think.

On 5 June 2014 13:59:04 GMT+01:00, "Mega [Andreas Stuermer]" <masterstorm123@gmail.com> wrote:
Hi everyone.

Anyone got an idea how to freeze Wisdom teeth cheaply? I asked at a in-vitro-fertilization bank and at the Red Cross. The first said "absolutely no" while the second one said "rather no" perhaps quick and dirty, and 100€ per year.
 
Isn't there a cheaper way? What if I use -20°C? The cells probably won't be functional after few months right?
 
Any input appreciated ;)


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