Re: [DIYbio] Re: Low cost PCR(under development)

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There are two ramp rates; ramp while increasing, and ramp while
decreasing. Most machines can achieve 3-4C increasing because they just
use heating coils, but their decreasing temperature (accomplished with a
peltier, cooling fluid, etc.) may be lower.

IIRC, OpenPCR has a climbing ramp rate of 2-3C, and a reducing ramp rate
of 1C. Larger machines with fundamentally the same architecture (coil +
peltier) achieve higher rates merely by adding extra wattage.

Remember though, an additional consideration is how homogenous the
temperature change is through the block, and at what rate the
temperature change in the block is reflected in the tubes. There would
be little point doubling the ramp rate of the block if the tubes cooled
at an only marginally faster rate. Worse, doubling the ramp rate as
measured by the thermometer may look great on the charts and benchmarks,
but the block may be unevenly cooling and this can have real
consequences when you're expecting your samples to experience identical
conditions.

It's rare that you'll need decreasing ramp rates faster than 1C, in any
case. Have you a special experiment in mind?

On 30/06/14 10:59, Michael Shamberger wrote:
> OpenPcr has a ramp rate of 1 degree C/s. I checked specs for some
> commercial versions and they have ramp rates of 3 or 4 degrees C/s. What
> are they doing differently?
>

--
T: @onetruecathal, @IndieBBDNA
P: +353876363185
W: http://indiebiotech.com

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