I Have been designing a PCR machine for a month ago. Now the designs complete and have completed the analysis.The machine is working properly but the intensity of amplified bands are too low.The machine was cross tested with a Bio Rad machine in my department laboratory and was analysed in UV it gives some unwanted amplification also.My theory was right i was actually able to make the machine work.Now going for the secondary design having a peltier element and a new mechanism for faster cooling and heating
-- "The results of my Machine is attached as a picture with it"........."Please see it and give your suggestions"
Subin
Dept Of Biotechnology
National institute of technology Calicut
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