So I tested a few different medias. I think the problem was that I was using SOC. I thought using a more defined, nutrient rich media would help but instead it does the opposite. You can't really see any glow. I also found out that BL21 cells glow much better than DH10B.
So using pJE202 pVIB with LB Amp and BL21 works best for me so far.
Going to try some other stuff before cloning into a overexpression plasmid.
On Sun, Jul 20, 2014 at 2:01 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
Cool thanks.On Sun, Jul 20, 2014 at 1:08 PM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
It's long ago I tried this. Much LB -> much glow. It was visible nicely, but the camera didn't like it. Plus you had to open the cover before taking a photograph due to oxygen depletion.
On Sunday, July 20, 2014 8:45:51 AM UTC+2, Josiah Zayner wrote:Has anyone tried and been successful to see pJE202 or pVIB glow in liquid culture in E. coli?Thanks,
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