Hmm seems like theory getting in the way of practical. Cooperativity refers to number of cI binding sites, either 2 or 3. But you should find that repressor is temperature sensitive dimer (cI857 allele) and falls apart above a certain temperature. In which case, heat pulse causes transcription. If wild type repressor, repressor will be under IPTG induction (ptac or plac) remove IPTG and the repressor levels will begin to drop and expression from pI will begin.
>matt
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Zhen Zhang <izgzhen@gmail.com> wrote:
For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.
-- So I suppose that I should use Hill Kinetics to model this regulation process.
So I need three parameter to describe the regulated transcription rate
1. Hill coefficient or the cooperativity constant n.
In the Wikipedia, it give two conditions with n = 2 and n = 3.
2. Dissociation constant Kd which I don't know
3. The Maximum Transcriptional Rate Vm which I also have no idea of.
Where should I go to find the later two parameters on web?
Is there any database that you always use for such condition?
I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me!
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