Re: [DIYbio] How to get certain biochemical data?

For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.

So I suppose that I should use Hill Kinetics to model this regulation process.

So I need three parameter to describe the regulated transcription rate

1. Hill coefficient or the cooperativity constant n.

In the Wikipedia, it give two conditions with n = 2 and n = 3.

2. Dissociation constant Kd which I don't know

3. The Maximum Transcriptional Rate Vm which I also have no idea of.

Where should I go to find the later two parameters on web?

Is there any database that you always use for such condition?

I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me! 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAPdu10jUPqK2dNLoy5bnvOw4-aJsVKVWwLwkTGuuNaKEMb7wpg%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.