Yeah, the protein is unknown. We just know that one of the 10'000 protein of Coptis contains this enzyme that modified berberine.
Maybe it would be able to bind berberine to a column and thus bind the broteins?!?
Maybe it would be able to bind berberine to a column and thus bind the broteins?!?
On Fri, Aug 1, 2014 at 3:06 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
I'd have suggested affinity chromatography, but it sounds like they're
on a "fishing expedition" and don't know precisely which proteins they
want, yet..
On 01/08/14 14:04, bioscisam wrote:
> You could try something like His tagging where you add a few histidine
> residues to the end of your protein (i.e introduced in your expression
> vector) and then isolate on a nickel column such as HiTrap from GE
> Healthcare, there's probably cheaper suppliers of alternatives.. These come
> as mini syringe versions for small preps rather than Acta scale protein
> prep systems.
>
>
> On Friday, August 1, 2014 11:01:53 AM UTC+1, Mega [Andreas Stuermer] wrote:
>>
>> Did a quick google research on it, no protocols found so far. How can I
>> immagine dialysis step?
>>
>
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