Re: [DIYbio] Re: DIY Protein Separation

Might be possible to separate them on/through sepharose if there's a
non-denaturing protocol. As for binding berberine to the column sounds
like an interesting approach although not sure of the chemical
reactions required, an organic chemist will definitely know more. Most
crosslinking kits seem to be for linking standard things like biotin
and streptavadin to things like oligos and antibodies, but am sure
there's a way to do it.
If resources/funds weren't a limiting factor I would try to assess the
activity of FPLC fractions, then LC-MS on those fractions to try to
identify protein sequences, then design degenerate primers to fish
their genes out and try to clone and express... I've never seen a DIY
mass spec though! One day maybe...
Otherwise sometimes you can get access to university central
facilities if you are associated with them or send to a CRO for
testing...
Basically these are the paths I'd want to one day see DIYBio work
around but currently I think that's the typical approach.


On Sat, Aug 2, 2014 at 8:57 AM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> What about gel chromatography for proteins? It would separate them by size.
>
> And seems relatively do-able?
>
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