Re: [DIYbio] Karyotyping? etc

Jem:

I'm interested in this as well. I think we need to come up with an open protocol for this. I assume the limiting step is a fluorescence microscope, which is $$$$, but this would make an interesting DIY build and application, even if just for simple curiosity. 

I think Eric Harness has been working on a fluorescent microscope at BioCurious, I have cc'ed them, correct me if I'm talking out of school.

Sorry for the list </aol> but mostly this is a "me too".

Matt Harbowy -hbergeronx@gmail.com
650 243 8467 - @hbergeronx on Twitter

On Sep 22, 2014, at 2:22 AM, jem <jemmaredmond@gmail.com> wrote:

H!

So this is a slightly unusual post. But I was wondering if anyone could offer any advice?

To get to the point, I appear to have been born with an intersex condition, I've had a number of hidden surgeries, etc and I'm still unraveling a bunch of lies having been told to me by the medical profession (and this happens a lot to other's too).

Anywho, I'm looking to do karotyping on myself and perhaps some others... and I was wondering what is the procedure for doing this? I've had a lot of frustrations and difficulties going through the local health service to get these tests done, mainly because they simply don't want to do it as it costs money and is probably deemed by most doctors or endros to be a waste of time and indeed that can be the case, (they also generally don't have a clue about dsds/intersex conditions).  I actually work in a biomedical facility but I'm a physicist primarily, I dabble in tissue engineering growing cells on scaffolds, etc. So I've been asking around here and in the medical school about getting these tests done but I've been told its not so easy, contamination, etc etc 

So does anyone know of any papers / procedures for doing these tests? I have access to every type of equipment here. :-)

Ta

Jem



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