RE: [DIYbio] Re: Fish gDNA isolation

Would love to see the protocols in that paper but..paywall. :(

On 30 September 2014 18:11:01 GMT+01:00, scocioba@gmail.com wrote:
Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Yuriy Fazylov
Sent: ‎9/‎30/‎2014 9:54 AM
To: diybio@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation

Try Sambrook et al (1989) I haven't read into it personally.
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a.html
Trying to extract GFP from glow fish?

--
Sent from my Android device with K-9 Mail. Please excuse my brevity.

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