RE: [DIYbio] Re: Fish gDNA isolation

Perfect! Thanks! I'll post results of the workshop to the list if it works well...in case anyone is interested.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Yuriy Fazylov
Sent: ‎9/‎30/‎2014 9:26 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation

http://primerdigital.com/dna.html (bottom of the site)

On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:
You learn something new everyday! I didn't know tilapia was bred like that. Thanks for the factoid. I was leaning more towards tuna and salmon since Atlantic and pacific have varied prices.

Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Yuriy Fazylov
Sent: ‎9/‎30/‎2014 8:28 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation

Cool. I didn't expect the link to be spot on. 

If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger. 

On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:
Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Yuriy Fazylov
Sent: ‎9/‎30/‎2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation

Try Sambrook et al (1989) I haven't read into it personally.
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a.html
Trying to extract GFP from glow fish?

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