Amplify the gene in question and use a quantitative realtime PCR assay and look for differences in high resolution melt curve vs control.
Or, drink 3 glasses of milk, wait a couple months on lactose free diet, and drink some of the lactose-free stuff. Then analyse your feelings? That is the old school way, the gut feeling. That and tallying those in your lineage who have it, and arriving at a conclusion that is, if not empirical, at least probable in a Bayesian sense.
I would recommend the 23andme sequencing, I got mine done. As long as you are comfortable with the reported error rate- which could be diminished with repeat sequencing and some sort of alignment algorithm. Otherwise you are talking a lot of work and access to expensive hardware.
On Tuesday, October 21, 2014 2:22:42 PM UTC-7, Mike Petersen wrote:
Hi everybody,
let´s say I would like to do a genotyping at home. Does anybody know how to do that?
Is there anything like a lab protocol?
So for example I´m looking for a special SNP like the lactose intolerance SNP (http://www.snpedia.com/index.php/Rs4988235 )
I know, I could do a test with a gene chip with a company like 23andme.
But just for my interest: Is there any way to find out a special SNP in the DIY-way at home?
Get the DNA, isolate it, do the PCR to amplify but what to do next?
Thank you in advance
Mike
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