RE: [DIYbio] SNP Genotyping at home - lab protocol?

You need to sequence the gene. Sanger is a cheap way to do that, but you will have to look for some company. After that, you compare your sequence withsome database. But to be sure about theresults, you have to use controls during your dna extraction and pcr amplification, and check them also by sanger.
Before Sanger, remember that you have to clean your sample.

If the mutation that you are looking for is enough big (lets say normal gene size is 1kb and mutated gene size is 0,7kb) you could check it by agarose electrophoresis, after PCR. But is not the usual case in human genetics

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/ed16cb41-0414-4b52-89e7-6044e1dd9a1a%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.