Re: [DIYbio] reverse osmosis systems for autoclave and microbial purposes



On Wed, Oct 29, 2014 at 5:32 AM, Tom Hodder <tom@limepepper.co.uk> wrote:
Hi all,

We recently obtained a melag euroklav off ebay, and it's brilliantly easy to use, but it's using a lot of our stock of deionized water.

I am thinking about proposing we get a reverse osmosis system for the lab, something like this;

I was wondering whether anyone had any comments, or experiences of RO filter systems for use in a microbial lab..


I don't know too much about RO systems, but years ago I helped install one on a farm/ranch where they had spring water. Since this was in a semi-arid climate, and thus water was a limited resource, there was concern that the filters used a lot of water to backflush and clean them out. I bring this up as I wonder if you would need to backflush with sterile water, to prevent contamination from lodging itself onto the clean side of the filter, leaving a looming latent contamination problem.

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