[DIYbio] Re: Isolating sequences from scratch

>Then design primers to isolate the gene
 
I think when the OP stated he wanted to learn to isolate the gene, what he may have meant was to determine which gene was reponsible for that particular trait.  That's a bit trickier.  I would suggest generating a complete genome sequence followed by some (a lot) of analysis of the data.  Keep in mind that the translucency you are interested in is probably the result of a bunch of genes.  Also keep in mind that plant genome sequencing requires a healthy budget for the libraries/sequencing.  You can start off small though, then continue as resources allow.
 
Neat project, and beautiful plant.
 
 

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