[DIYbio] Re: Isolating sequences from scratch

SC, Undoubtedly so.

How would you recommend starting, learning, and working through the process? and what sort of budget are you implying for a project of this size?

I'd love to see this artistically in ornamentals as well as utilizing this as a 'sensor.'
On Monday, November 24, 2014 4:21:31 PM UTC-8, SC wrote:
>Then design primers to isolate the gene
 
I think when the OP stated he wanted to learn to isolate the gene, what he may have meant was to determine which gene was reponsible for that particular trait.  That's a bit trickier.  I would suggest generating a complete genome sequence followed by some (a lot) of analysis of the data.  Keep in mind that the translucency you are interested in is probably the result of a bunch of genes.  Also keep in mind that plant genome sequencing requires a healthy budget for the libraries/sequencing.  You can start off small though, then continue as resources allow.
 
Neat project, and beautiful plant.
 
 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/4b35a56b-458f-4c60-aab2-5cc0b34831c3%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment