Re: [DIYbio] Re: Isolating sequences from scratch

Probably tens of thousands.  How deep have you dug into the literature to see if there are any experts on this plant?   You might find crazy botanist who is obsessed with it, though someone may have never published papers on it if it hadn't been used in natural medicine or something of that nature.

To be honest, to me it seems like it's more of a physics question than a biology question, if that even makes sense.  Think about getting white paper wet, it appears translucent because of differences in indexes of refraction etc.     I don't recall all the necessary physics, optics, and waves equations to explain it, but with some reading or another explanation from someone on here more versed in that field, it might make sense.

If you can't find specific genes for this plant which I imagine might be hard since it's not a model organism, you could at least explore another flowering plant with white petals, and learn about the biology of that, including the formation of its petals, pigmentation (or lack there of)


Mesophylls n things?  

It is odd looking...but very pretty.  I wonder if there was an evolutionary reason for it.  There are no doubt tons of papers published on genes encoding petal pigmentation, but I think the ultimate question is:

Is there a chemical reaction going on causing the pigmentation in the petals to vanish, or is the design of the flower in terms of thickness and other physical parameters just right at which point it appears translucent upon getting soaked with rain water?   I'd go with the latter.

"The plant can be found growing on moist, wooded mountainsides in the colder regions of Japan and China come late spring."  Maybe there is a reason for that based on the environment in which it grows?  Maybe there is some special insect drawn to it for some reason more so when the petals are white or clear? 



It's so cool all those insane words are used to perfectly describe the plant.  One little organism!  Now you got me side tracked reading about flowering plants in the hills of Japan.


On Mon, Nov 24, 2014 at 10:04 PM, Nico B. <perpetuatetheimaginary@gmail.com> wrote:
SC, Undoubtedly so.

How would you recommend starting, learning, and working through the process? and what sort of budget are you implying for a project of this size?

I'd love to see this artistically in ornamentals as well as utilizing this as a 'sensor.'

On Monday, November 24, 2014 4:21:31 PM UTC-8, SC wrote:
>Then design primers to isolate the gene
 
I think when the OP stated he wanted to learn to isolate the gene, what he may have meant was to determine which gene was reponsible for that particular trait.  That's a bit trickier.  I would suggest generating a complete genome sequence followed by some (a lot) of analysis of the data.  Keep in mind that the translucency you are interested in is probably the result of a bunch of genes.  Also keep in mind that plant genome sequencing requires a healthy budget for the libraries/sequencing.  You can start off small though, then continue as resources allow.
 
Neat project, and beautiful plant.
 
 

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