[DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

hello friends, 
 i respect your gathered concerns and there is nothing to get alarmed about mammalian cell culture until it's being used up on human beings ...
    and i thoroughly understand the intention behind cautioning me.. i am actually feeling very encouraged and  will be continue to pursue my passion ....
    now i am actually looking into adaptable  approaches  to produce Recombinant Factor VIII using pichia pastoris is a species of methylotrophic yeast. 
    Pichia is widely used for protein expression using recombinant DNA techniques.
    i sincerely believe that you ppl and Diybio will continuously advise me  in this regard and guide me to accomplish my goal rather than plotting the scopes of Diybio 

    "If your actions inspire others to dream more, learn more, do more and become more, you are a leader...."
  
   and in my view Diybio is a leader and savant ppl like you made this group a leader in all aspects ...

   friends pls through some light on pichia pastoris and its pros and cons 

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