Re: [DIYbio] I want to DIY a Stirred-Tank Bioreactor

I have to drop out of the woodwork to agree with leaking pen.  I honestly thought the last 3 posts I saw to this group were trolls.  One wanted to do the Haber process in their kitchen, the second wanted a multi billion dollar biologic drug delivered in their garage, and the third asked for everyone on the list to do everything for them.  While I also can understand the after-posts, I can relate to the utter frustration of certain types of "gimmie gimmie gimmie" questions from people we don't know.  Some level of initiative should be shown, or people shouldn't be surprised by the type of responses they get.  Language plays a barrier, yes, but not in this particular instance I don't think.

I think now I understand why some of my professors just utterly snapped once in a while on the class as a whole.  I assume from their perspective their was this undeserved entitlement to laziness from some students, and if they all didn't get A's, it was the professors fault, when in actuality very few people overcame a challenge when met with one.

So, I'd say I completely relate to leaking pens frustration, and I'd also side with this being an "open and friendly list" but I don't see the need to call people out on it to seem morally superior in some way.   

If you ask a shitty question, expect a shitty response.

On Wed, Jan 21, 2015 at 7:33 PM, Erico Perrella <erico.perrella@gmail.com> wrote:

Here (http://bit.ly/1xZuoaY) you can find a little bit of theory behind bioreactors.

Attached you can find some papers containing more detailed information about how to actually build them.

If you are interested in photobioreactors, then this website http://algaegeek.com/ have some nice projects you can take a look.

You can ask me directly in my email if you have any doubt, I am happy to help if I can.  

:)

On Wed, Jan 21, 2015 at 10:32 PM, Erico Perrella <erico.perrella@gmail.com> wrote:

Here (http://bit.ly/1xZuoaY) you can find a little bit of theory behind bioreactors.

Attached you can find some papers containing more detailed information about how to actually build them.

If you are interested in photobioreactors, then this website http://algaegeek.com/ have some nice projects you can take a look.

You can ask me directly in my email if you have any doubt, I am happy to help if I can.  

:)

On Wed, Jan 21, 2015 at 5:23 PM, Otto Heringer <ottowheringer@gmail.com> wrote:

Assuming that is true she's being lazy or something, why botter!?

I just cannot understand someone activelly getting annoyed, spending all his ATPs to read and write something, just to express how annoyed he is.

How could you be so sure she is playing a intelectual dishonesty? Do you know her?

I hope to see the day when programming and electronics is a massively popular thing between life scientists - and when I say massively, I say in all the world, including countries in development. Im sorry Pen, but you just assumed this a commom thing, and is not.

Git hub is a messy repository for the one not used to it (there is even a nice initiative to teach how git works and how to use it - http://software-carpentry.org/).

A well documented source of information in a more friendly approach is the hackteria.org wiki. You shoul take a look. See, for instance, this page about a microalgae biorreactor: http://hackteria.org/wiki/Algae_Culture_at_Home

If you build this with your local materials and resources, please also share here the results <sarcasm> just to show how lazy you are </sarcasm>.

Free intolerance makes me mad.

Em quarta-feira, 21 de janeiro de 2015, Mega [Andreas Stuermer] <masterstorm123@gmail.com> escreveu:

I think it's ok when fresh people want to learn science. Some of course will have no clue yet. But we shouldn't condemn them. Actually one causes intolerance against  diybio and science in general by repelling people. 

I also had no clue of practical biotech before getting the keywords here needed, and collecting the knowledge where to search for infos. Then I collected a decent amount of knowledge enabling me to design own experiments (with more or less success, strongly depending on my private budget situation) way before getting educated in University. And frankly, University didn't teah me much new biotech knowledge (just the enzymes in greater detail. And of course getting my hands dirty on wetware)

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