i welcome your suggestion Filip ... the idea is not to produce the protein to administer to human beings the idea is to go through the entire cycle of recombinent protein production.. just decode the mistress of mammalian cell culture.. basically my philosophy in life is "the person how knows exact direction to his destination can never fork or hack the system" ... i know i am lost but in the detour i may find some thing which wouldn't have notice in actual thought-up path...
but Filip i really understand your concerns and i will be looking forward for future advices too fro you
On Fri, Jan 23, 2015 at 5:02 PM, Filip Hasecke <filip.hasecke@googlemail.com> wrote:
FilipCheersI like your ambition as a DIY biologist, but the preparation of clinical/pharmaceutical therapeutics is far beyond the scope of a DIY project.Those things aside. Unless you are a really rich person, the costs of the equipment that is required for the expression, purification, packaging and storage steps is incredible. You would need a professional clean room to prepare your samples, pharmaceutical grade chromatography equipment, viral filtration, just to name a few...In our lab we also work on mice and drug administration of protein therapeutics. Hence I know about the high standards that need to be fulfilled to even keep your mouse alive.. ^^Hi Sunil,sorry to be the fun killer, but you should forget about home producing clinical-grade protein therapeutics. Slight contaminations or aggregation of your protein might lead to immunogenic reactions towards your therapeutic. And a second administration of your protein, after the patient developed anti-therapeutic antibodies, might be lethal in the worst case.--2015-01-23 1:42 GMT+01:00 Sunil Phani <consultsunilphani@gmail.com>:For bacteriaOn Fri, Jan 23, 2015 at 3:34 AM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:You mean a plasmid? For bacteria or plant?
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