Re: [DIYbio] Re: low cost positive pressure laminar flow HEPA filtered box

On Tuesday, January 27, 2015 at 4:54:22 PM UTC-8, Otto Heringer wrote:
There's also this well documented tutorial. The results looks nice.

That one is definitely not a laminar flow hood though. For laminar flow, you want the entire air mass to move as one smooth sheet towards you (or form the ceiling to the floor, in vertical laminar flow). 
 
Because the Hackteria design only has one small cylindrical filter, the filtered air will flow out from there with lots of turbulence, and that turbulence can suck in contamination from the front of the hood. There's also no way they can match the recommended air volume of 100ft/min with a filter that small. 

The professional and DIY laminar flow hoods I've seen typically have a large rectangular HEPA filter that takes up the entire back surface of the hood, allowing the entire air volume to move towards you in one uniform mass. One of the links I posted above suggests how to check for laminar flow with a lighter: the flame of the lighter should be moving straight out away from the filter without any flickering whatsoever. That's a LOT of airflow!

Patrik

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