Re: [DIYbio] Somatropin

For high quality amino sequence don't trust Wikipedia, go directly to
uniprot.org. Prefer gold-starred sequences, and feel inspired to read
the references, if any. Get to know your protein; does it need
post-translational modification? Is there a signal peptide that needs
cleaving? Does it get glycosylated? Does it need help folding? Are there
cysteine for disulphide bridges?

On 26/01/15 04:16, Dakota Hamill wrote:
> HGH is another expensive biologic, and probably a grey area drug, since
> it's so often associated with steroid use.
>
> Things like HGH can save someones life, or destroy it. Hormones are
> pretty powerful and their chemistry is quite interesting, especially
> since the timing of their release and the dosages have such a profound
> affect upon the body and mind.
>
> That said, google would give you everything you need to know. Wikipedia
> will probably even give you the amino acid sequence, and i'm sure the
> DNA sequence for the protein is published. I don't think you'll be
> able to express it in E. Coli and inject it to get huge though.
>
>
>
>
>
> On Sun, Jan 25, 2015 at 12:06 AM, Aaron Chapman <redneckchappy@gmail.com
> <mailto:redneckchappy@gmail.com>> wrote:
>
> Can someone point me in the direction on how to synthesize
> Somatropin? Or at least where to start researching?
>
> Sent from my iPhone
>
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