[DIYbio] Best storage methods for strains in -20 freezer?

Hi all,

The gold standard for long-term storage of strains is glycerol stocks in a -80 freezer. Or even an ultra-low at -150C, or liquid nitrogen at -196C.

However given that most DIYbio labs don't have access to those kinds of resources - what are the best recommendations for storage in a -20C freezer?

50% glycerol? 15% glycerol? 10% skim milk? Stab cultures?

Opinions / best practices wanted!

Patrik

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