Hey all,
I'm working on a project where it would be extremely useful to have bacteria (E. Coli specifically) exocytose proteins continuously, rather than grow a culture and lyse it the old fashion way. I've found a few papers on this so far (1, 2, 3), but they are all for Gram-positive bacteria, which E. Coli is not. If need be, I could switch over, but it would be nice to not have to. I do have some information the Sec pathway in Gram-negative bacteria, but it is somewhat general.
Anyone have an experience/insight into this?
Thanks,
Jake
-- I'm working on a project where it would be extremely useful to have bacteria (E. Coli specifically) exocytose proteins continuously, rather than grow a culture and lyse it the old fashion way. I've found a few papers on this so far (1, 2, 3), but they are all for Gram-positive bacteria, which E. Coli is not. If need be, I could switch over, but it would be nice to not have to. I do have some information the Sec pathway in Gram-negative bacteria, but it is somewhat general.
Anyone have an experience/insight into this?
Thanks,
Jake
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