Hey Guys
First of all sorry for my bad English. I`m a beginner and need some help about plasmid.
So you can use plasmids for cloning (to copy a DNA-Part many time in a Bacteria), but also as a vector for gene transfection or transformation is that right?
So for Example, can I design a Vector and put it with for example with electroporation in the cell, and the Cell would, if I had de right Promoter, transcript the DNA and produce the Protein that I had on the Vector?
If that's right, I had two questions…
1 How can I bring the Vector that is in the cell plasma because of de Electroporation into the Genome of the Cell?
2 I have seen the website http://www.addgene.org/ there it gives many plasmid. Are this also vectors? And in there Plasmid it has Promoters, Parts for restriction enzyme and ORFs. But is in the ORFs already some codes for Proteins or must I add this? And can I find there a vector for plants?
Sorry for my questions but I am a beginner and found not very much information on the internet.
Louis
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