i may be sounding obnoxious but why can we hack a typical UV & RO (ultra violet and reverse osmosis ) water purifier off the shelf
-- to achieve protein purification ... coz every high end water purifiers now a days promises to be having chromatography and filtration
i wonder if there is already a DIYBIO chromatography using off the shelf UV & RO water purifier coz
every other UV & RO water purifier discusses deeply how it separates organic and inorganic
solvents and compounds from water.. then just as appetite to brain why cant we give it a
thought on its merits
pros
- every water purifier have chromatography and filtration mechanism
- every water purifier have pH, UV sensors, but i don't know weather they have conductivity sensors are
- every water purifier have filtration mechanism ( may not be 2 µm)
- every water purifier have pH, UV sensors, but i don't know weather they have conductivity sensors are
- most of the UV & RO water purifier have column chromatography (suitable resin is arguable)
- most of the UV & RO water purifier have ion exchange chromatography (but we need to hack it, like we were hacking Hp inkjet printers to print 3d microbial cells )
- needs some reverse engineering to achieve clarified lysate purification
- needs some reverse engineering to achieve HIC by adding ammonium sulphate etc, in recursively cycles
- most of the UV & RO water purifier have TFF but we need to hack permeate and retentate chambers and focus in recycling renantate several time through diafiltration for efficiency
guys this needs some kind of hacking to off the shelf UV & RO water purifier pls give it a thought and come up with your ideas and viewpoints on this topic.,
becausehow often can every DIYBIO labs can afford such whopping expensive proprietary chromatographys lets rip open an opensource platform for purifying
proteins using off the shelf purifiers
"No problem can withstand the assault of sustained thinking. ~Voltaire"
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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