Hi Sunil,
It's always good to think of new ways to do things.
In this case though, remember that by "protein purification" what you describe is purification of a mixture of all the proteins in the culture. But what you'd really need to do is purify a single protein and separate it from all other non-target proteins.
Different types of proteins have different purification methods. The more similar the target protein is to the non-targets, the more difficult the separation. You may want to check into chromatography or epitope fusions.
Stacy
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