guys help me to choose between CHO or Pichia pastoris or AAV plasmid to synthesize Recombinant Factor VIII on the merits of efficiency ,time and money ,
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
-- because to express polynucleotide sequence split into heavy and light chains of Factor VIII in Pichia pastoris expression system is just workaround and will defiantly consume lot of time and obtaining identical sequence for a large protein as long as ~220kDa, 2,351 amino acid is not going to be easy ... where as mammalian expression systems is used extensively till date either with CHO or HEK hence we dont need much of the rework and don't consider mammalian culture as taboo coz its just in vivo culture,,
expecting u guys valued opinion soon /.......
Regsrds
Sunil Phani
consultsunilphani@gmail.c0m
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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