Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII


On Feb 7, 2015 11:28 AM, "Sunil Phani" <consultsunilphani@gmail.com> wrote:
>
> guys help me  to choose between CHO or Pichia pastoris

Well CHO you're gonna need serum and antibiotics (likely) and a CO2 incubator, with Pichia you'll need a supply of methanol. I can't remember details of growing Pichia but it seems like if you can get a culture, it could be easier to grow.... But whether it will express correctly compared to CHO is hard to say.

I take it you've seen this:
http://www.ncbi.nlm.nih.gov/pubmed/16960007

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