In my experience, RFP is more visible than GFP under normal lighting conditions. May want to use that instead
-- -Koeng
On Tuesday, March 17, 2015 at 3:01:36 PM UTC-7, Josiah Zayner wrote:
On Tuesday, March 17, 2015 at 3:01:36 PM UTC-7, Josiah Zayner wrote:
Most GFPs I have worked with make bacteria look green under normal lighting conditions. It more depends on the quantity of the GFP than having a specific GFP. Same is true for RFP and the likes.
On Tuesday, March 17, 2015 at 9:00:03 AM UTC-7, Mega [Andreas Stuermer] wrote:Thanks! You are right it seems.
http://parts.igem.org/Part:BBa_I746916 Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP.
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