Re: [DIYbio] Re: mutant GFP - green phenotype of E coli

Most GFPs I have worked with make bacteria look green under normal lighting conditions. It more depends on the quantity of the GFP than having a specific GFP. Same is true for RFP and the likes.

On Tuesday, March 17, 2015 at 9:00:03 AM UTC-7, Mega [Andreas Stuermer] wrote:

Thanks! You are right it seems.
http://parts.igem.org/Part:BBa_I746916

Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/P2i39ueT950/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e10d8a7d-8d79-4fc6-b6a4-a44f2b6fd399%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.