Hello,
-- I am studying Electronic Bioengineering at University of Bologna. I am beginning to study the working principle of qPCR, more specific I am now focusing on SYBRGreen. I would like to do some optical tests on that chemical compound. My biological knowledges are not excellent, so I thought that my first step could be finding a datasheet of this subastance. I did several researches on google, but I can't find any documents where the behavior of the SYBRGreen is completely described. I would like to know:
- emitted light / incident light
- wavelenght of the emitted light
- sensibility / wavelength of the incident light
- changes with temperature (?)
So I would like to have a datasheet, something that describes SYBRGreen entirely.
Where can I find this document?
Thanks and regards,
Gabriele
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