Hi, I would like to setup a small lab to do some beginner's work. Currently I have no biological equipment, but I am able to work on electronics and I work as a software developer. I think it is probably best to grow the lab in several phases. First I'd like to concentrate phase I but to keep all alter phases in mind, but only to use them when I have to decide which of several paths to follow. Phase I I'd like to be able to perform PCR and do some genetic fingerprinting/barcoding for plants and animals. Phase II I'd like to create my own GMO bacteria - probably starting with adding luciferase or something similar (as I understand the luciferase gene is patented) to E.coli Phase III GMO with plants Phase IV Work on c.elegans I am grateful for all feedback for all phases, but would really like to concentrate on phase I for now. I am located near Munich in Germany. I would be happy to take part in any courses that are open to non-students - but I don't know of any. The "Deutsches Museum" in Munich has a visitor's lab - I've done a PCR experiment there, but this is a 2 hour experiment and no followup is planned nor are deviations from the standard protocol possible. Also I'd be happy to join some kind of community lab, but again - I don't know any. All pointers are welcome. If there really is no place for me to work on these kinds of projects I would look into how to set this up myself on a budget. The first steps would be to build a thermocycler and a electrophoresis box. I'd probably buy a pipette (which size should I choose?) and a centrifuge - I have a lot of respect of fast-spinning-stuff and would not risk building something like this on my own. For the thermocycler I am not sure how fast is has to be able to heat up and cool. Especially cooling might make this more expensive, If I have to cool faster than a normal fan would do. Obviously the cycle time gets longer if the cooling is not as efficient, but are there any other adverse effects, if I cool more slowly? Also how exact do these temperatures have to be? Apart from these questions, I expect I'd be able to handle the equipment part on my own. But where can I source the chemicals? What kind of dye should I use (I'd prefer non-carcinogenic)? I hope you can help and give me some pointers! Thanks in advance Oliver
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