Check through some of my old blog posts on www.basementbiotech.org we barcoded a ton of fungi. Dried mushrooms are fine. We had 3 year old dessicated mushrooms we got ITS bands on with a simple ethanol prep.
On Jul 10, 2015 5:22 PM, "'peter russell' via DIYbio" <diybio@googlegroups.com> wrote:
-- I am a complete novice but want to do some DNA barcoding of fungi around where I live in New York. Am looking to visit my local community biolab but had a few basic questions. I have a herbarium of dried fungi .... am I likely to have any success with these or do I really need fresh specimens? Has anybody tried this on Waxcaps and if so what [cheap][simple] extract protocol was successful? If I am asking this in the wrong forum I apologise... if anybody can suggest an approriate forum I would appreciate it!--
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