Re: [DIYbio] DNA barcoding of mushrooms

If you don't know what you're looking at: Look up 28S or ITS2 barcode
sequencing.

Here is a preparation protocol, which would get any samples ready for
sending off for sequencing:
http://1ofdmq2n8tc36m6i46scovo2e.wpengine.netdna-cdn.com/wp-content/uploads/2015/02/iTag-Sample-Amplification-QC-v1.1.pdf

If you know what mushroom you're looking for, and there are
pre-existing primers for a unique-to-that-species gene-fragment, then
you may be able to get around sequencing and simply do a PCR.

On Fri, Jul 10, 2015 at 9:18 AM, 'peter russell' via DIYbio
<diybio@googlegroups.com> wrote:
> I am a complete novice but want to do some DNA barcoding of fungi around
> where I live in New York. Am looking to visit my local community biolab but
> had a few basic questions. I have a herbarium of dried fungi .... am I
> likely to have any success with these or do I really need fresh specimens?
> Has anybody tried this on Waxcaps and if so what [cheap][simple] extract
> protocol was successful? If I am asking this in the wrong forum I
> apologise... if anybody can suggest an approriate forum I would appreciate
> it!
>
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--
-Nathan

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