[DIYbio] What do you think of this idea?

5:17 PM |

Hi Andreas,
I have an idea. Can we make this a DIYbio group effort and release the sequence data to the public? As I am reading on this, it looks like using Nanopore is not easy. Especially, the alignment and computational stage looks daunting to me. I also am busy with other projects and time is short. My proposal is below (I want to sent to DIYbio mailing list, but I need your approval before I email it). So please tell me if it is OK to email the DIYbio group?
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Idea: Community effort genome seq?

Andreas, our friend on the list, recently asked about using the Oxford Technologies Nanopore. I reached out because the lab I work for does have a Nanopore MiniIon. He proposed sequencing the Panellus stipticus genome to determine how to synthesize the fungal luciferase. I don't know if the sequence is publicly available or not. I like his proposal but I will have to convince the boss to carry this out. I don't really know how similar the Nanopore is to the Ion Proton NGS platform, in terms of cDNA library preparation or data analysis. It will be wonderful if the community came together to sequence an organism. I don't know how it can be done. If we can sequence the genome and make it public, I think it will be a big step for DIYbio. Time is of the essence for me.
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What do you think, Andreas?

Thanks,
Jeswin

--
In necessary things unity; in uncertain things freedom; in everything compassion.
-Marco Antonio Dominis

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