Re: [DIYbio] Amino bio

On 10/22/2015 08:55 AM, Koeng wrote:
> https://www.indiegogo.com/projects/amino-desktop-bioengineering-for-everyone#/
>
> Interesting idea. I find that I would most likely only use this for continuous evolution, based on the tools included.


The feature list is a good specification for a bioreactor for continuous growing, the switch
to another variety or species using its easy chemical inactivation that is automated.

The Amino One seems definitely "hand crafted" as they say, and with a high price tag to pay for that.

I've got a question for you diybioers: If you have a system of connections based on O-rings that are compressed
so they create surface seams that are not deep in grooves, how effective would H2O2 be if purging/sterilizing was
accompanied by higher than normal system pressure?

I am thinking the flexible O-rings would cave in some with the extra pressure, thus exposing more of the seam
crack to the sterilizing agent (H2O2 or other).

Flex tubing probably will expose more surface as pressure goes up if the tubing is push-on type of fitting.
Amino shows ordinary flex tubing pushed onto a clear fitting in video titled "RFP culture in Amino P2".
I wonder if that can really be sterilized enough with liquid disinfectant?

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