Presumably with placing two conductors at each end of the tray, switching these alternatively on/off would average out the movement of particle through the gel and also perhaps speed the separation in the gel run since the movement is a zig-zag through the matrix.
try reading some actual journals some time, like Electrophoresis, which has plenty of validated, published research articles on this subject around 1980 +/- 10 years.
You know, actually RTFM!!
try reading some actual journals some time, like Electrophoresis, which has plenty of validated, published research articles on this subject around 1980 +/- 10 years.
You know, actually RTFM!!
## Jonathan Cline ## jcline@ieee.org ## Mobile: +1-805-617-0223 ########################On 10/19/15 10:28 PM, PoeVA wrote:
Interesting idea Conner! I had no idea spark plugs had Pt. Also, good point Nathan about the point source causing problematic distortion in a 2D gel. I wonder if a decent electrical field could be created with an evenly spaced array of spark plugs (or small lengths of Pt wire). Somebody fire up some E field modeling* :)
-Brian
* COMSOL or ANSYS might be fairly turnkey... or, for the brave, Mathematica/Matlab/NumPy should do it. I haven't even Googled it (let alone Google Scholar), so forgive me if prior work on the electrode array front is laying in plain sight.
On Monday, May 27, 2013 at 4:57:24 PM UTC-4, Nathan McCorkle wrote:A point electrical source might drag all the DNA to the center of the gel
On May 27, 2013 6:46 AM, "Conner Berthold" <cmb4...@gmail.com> wrote:
I had an idea a few days ago about using platinum spark plugs as the electrodes. One could be placed at each end and the center used for the electrical in/outputs. I hope to be building a few boxes soon and would just thread holes for them. Another plus would be that they could be changed out easy if they ever corroded.
Any ideas?
-Conner
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